Wang Resin LL (100–200 Mesh): Versatile Acid-Labile Resin for Peptides with C-Terminal Acids
Wang Resin LL (Low Loading) is a polystyrene-based solid-phase resin functionalized with the classic Wang linker (p-alkoxybenzyl alcohol), widely used for Fmoc-based peptide synthesis. It allows for the production of C-terminal carboxylic acid (-COOH) peptides via acidic cleavage. The low loading (LL) capacity of this resin (typically ~0.2–0.4 mmol/g) makes it especially suited for the synthesis of long, aggregation-prone, or sterically hindered peptides, minimizing chain-chain interactions and side reactions during SPPS.
Specifications
Matrix: Polystyrene cross-linked with ~1% DVB
Linker: Wang (p-alkoxybenzyl alcohol)
Loading: Low loading, typically 0.2–0.4 mmol/g
Particle Size: 100–200 mesh (approx. 75–150 µm)
Compatible Chemistry: Fmoc/tBu strategy
Cleavage Conditions: Acidolytic (typically TFA-based)
Final Peptide Terminus: C-terminal free acid (-COOH)
Key Features
Low Loading Capacity: Ideal for synthesis of longer or hydrophobic peptides
Acid-Labile Wang Linker: Yields peptides with free C-terminal carboxyl groups
Fmoc-Compatible: Seamless integration into modern SPPS workflows
Improved Crude Purity: Reduces deletion sequences and aggregation artifacts
Flexible Use: Suitable for manual, microwave-assisted, and automated synthesizers
Applications
Peptides requiring C-terminal –COOH groups
Synthesis of long, hydrophobic, or aggregation-prone peptides
SAR studies and therapeutic lead development
SPPS method development where resin loading impacts purity
Organic molecule synthesis via solid-phase strategies
Cleavage Protocol
| Reagent | Conditions | Result |
|---|---|---|
| TFA (95%) + scavengers | 1–2 hours at room temperature | Peptide with –COOH terminus |
Handling & Storage
Store dry and protected from moisture and light
Swell prior to use in DCM, DMF, or other compatible solvents
Compatible with all standard SPPS protocols and instrumentation
For best results, use under inert atmosphere for long-term storage
Comparison Table
| Resin | Loading | C-Terminus | Best Use |
|---|---|---|---|
| Wang Resin LL | ~0.2–0.4 mmol/g | –COOH | Long/aggregation-prone peptide synthesis |
| Wang Resin (standard) | ~0.6–1.2 mmol/g | –COOH | General-purpose SPPS |
| Rink Amide Resin LL | ~0.2–0.4 mmol/g | –CONH₂ | Peptides with amide termini |
| 2-Chlorotrityl Resin | Variable | –COOH | Acid-sensitive peptides, mild cleavage |
Conclusion
Wang Resin LL (100–200 mesh) is a high-performance, low-loading resin ideal for synthesizing high-purity peptides with C-terminal carboxylic acids, especially when dealing with difficult sequences. Its reliable performance and broad compatibility make it a cornerstone in peptide synthesis laboratories
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